*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Modified Tryptone Soy Broth (mTSB)
Specifications |
|||||
---|---|---|---|---|---|
Brand |
NEOGEN Culture Media | ||||
Formula |
Enzymatic Digest of Casein |
17.0 g/L | |||
Sodium Chloride |
5.0 g/L | ||||
Dipotassium Hydrogenphosphate |
4.0 g/L | ||||
D(+)-Glucose |
2.5 g/L | ||||
Enzymatic Digest of Soy |
3.0 g/L | ||||
Bile Salts No.3 |
1.5 g/L | ||||
Mass |
500 g | ||||
Package Dimensions |
4.60 IN x 2.90 IN x 8.60 IN | ||||
Package Weight |
1.35 LB | ||||
Preparation |
1. Dissolve 33 grams of the powder in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium if necessary.
3. Autoclave at 121℃ for 15 minutes. Cool to 45-50℃ and add 2 vials of Novobiocin supplement X150. | ||||
Previous Item Number |
Product was previously known as LabM Product: LAB165 O157 Broth (MTSB) |
Modified Tryptone-Soy Broth has emerged as the medium of choice for the enrichment of E. coli O157:H7 in red meats and is incorporated into ISO/TS 13136:2012. This medium is not intended for use in the diagnosis of disease or other conditions in humans.
Concern regarding E. coli O157:H7 has grown due to the severity of the disease syndromes caused, and the increase in foodborne infection, so too has the need to optimize methods for its efficient isolation. Symptoms start with severe stomach cramps and
watery, bloody diarrhea, and a percentage of individuals infected will develop Haemolytic Uraemic Syndrome (HUS) leading to acute renal failure. In a comparison of four different selective broth media, MTSB was the most productive and
selective for the isolation of E. coli O157:H7. mTSB is made selective for O157:H7 by including bile salts in the dehydrated medium, and the addition of novobiocin supplement (X150).