1. Suspend 29.8 g of the powder in 1 L of purified water. Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Follow label directions specific for each medium.
4. Autoclave at 121°C for 15 minutes, or as directed on the label.
5. Store fluid thioglycollate media at 15-30°C. If more than 30% of the medium is pink prior to use,
reheat once (100°C) to drive off absorbed oxygen.
6. Test samples of the finished product for performance using stable, typical control cultures.