*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Sorbitol MacConkey Agar
Specifications |
|||||
---|---|---|---|---|---|
Analyte |
E. coli O157 | ||||
Brand |
NEOGEN Culture Media | ||||
Formula |
Enzymatic Digest of Gelatin |
17.0 g/L | |||
Enzymatic Digest of Casein |
1.5 g/L | ||||
Enzymatic Digest of Animal Tissue |
1.5 g/L | ||||
Sorbitol |
10.0 g/L | ||||
Bile Salts Mixture |
1.5 g/L | ||||
Sodium Chloride |
5.0 g/L | ||||
Neutral Red |
0.03 g/L | ||||
Crystal Violet |
0.001 g/L | ||||
Agar |
13.5 g/L | ||||
Grams per Liter |
50.00 | ||||
Liters per 500g |
10.00 | ||||
Mass |
500 g | ||||
Package Dimensions |
4.60 IN x 2.90 IN x 8.60 IN | ||||
Package Weight |
1.35 LB | ||||
Plates per 500g |
560.00 | ||||
Preparation |
1. Suspend 50 grams of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
3. Autoclave at 121ºC for 15 minutes.
4. Cool to 45-50ºC.
5. If required add 2 vials of Cefixime Tellurite supplement X161 and mix well before dispensing.
| ||||
Previous Item Number |
Product was previously known as Acumedia Product: 7320 MacConkey Agar with Sorbitol; and LabM Product: LAB161 Sorbitol MacConkey Agar |
Sorbitol MacConkey Agar is used for the isolation of pathogenic Escherichia coli in a laboratory setting. Sorbitol MacConkey Agar is not intended for use in the diagnosis of disease or other conditions in humans.
Sorbitol MacConkey Agar is based on the formula by Rappaport and Henig. Originally developed for isolating enteropathogenic (EPEC) serotypes, O11 and O55, this medium is recommended for the isolation and differentiation of enterohemorrhagic E. coli O157:H7.
Serotype O157 has been implicated in serious foodborne diseases.
MacConkey Agar W/ Sorbitol contains sorbitol instead of lactose for differentiating enteropathogenic E. coli serotypes; these strains are typically sorbitol negative. MacConkey Agar W/ Sorbitol is recommended for food testing.
Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, and Enzymatic Digest of Animal Tissue are the nitrogen and vitamin sources in MacConkey Agar W/ Sorbitol. Sorbitol is the fermentable carbohydrate; typically enteropathogenic strains produce colorless
colonies. Bile Salts Mixture and Crystal Violet are the selective agents, inhibiting Gram-positive cocci. Sodium Chloride maintains the osmotic environment, and Neutral Red is the pH indicator. Agar is the solidifying agent. The selectivity
of the medium can be increased through the addition of Cefixime and tellurite.