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CONTENTS (Liter) | Tryptose | 10.0 g |
Beef Extract | 5.0 g | |
Yeast Extract | 5.0 g | |
Sodium Chloride | 20.0 g | |
Disodium Hydrogen Phosphate | 9.6 g | |
Monopotassium Phosphate | 1.35 g | |
Esculin | 1.0 g | |
Lithium Chloride | 3.0 g | |
Final pH = 7.2 ± 0.2 at 25°C. |
Suspend 54.95 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of Fraser Listeria Broth supplement A (MB-F1828) and 2 vials of Fraser Listeria Broth supplement B (MB-F0803). Mix well. Pour into tubes
1 vial contents (each vial is sufficient for 500 mL of medium) | |
Ferric Ammonium Citrate | 0.25 g |
1 vial contents (each vial is sufficient for 500 mL of medium) | |
Acriflavine HCl | 0.0105 g |
Nalidixic Acid | 0.01 g |
Fraser Listeria Broth is a medium used for the isolation and identification of Listeria spp. Tryptose, beef extract and yeast extract provide carbon and nitrogen sources and the cofactors required for good growth of Listeria spp. The high concentration of sodium chloride in the medium inhibits the growth of enterococci. Disodium hydrogen phosphate and monopotassium phosphate are the buffering agents. Listeria species hydrolyze esculin. Selectivity is provided by lithium chloride, nalidixic acid and acriflavine HCl. Ferric ammonium citrate is used to detect sulfite reduction. Fraser Listeria Broth except ferric ammonium citrate can be used for the cultivation of Listeria spp.
Inoculate the specimen using a sterile needle to the medium. Incubate at 36 ± 1°C for 24 - 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: beige
Prepared medium
Appearance: slightly opalescent
Color: light amber
Incubation conditions: 36 ± 1°C / 24 - 48 hours
Microorganism | ATCC | Growth | Characteristics |
Listeria monocytogenes | 15313 | good | blackening |
Staphylococcus aureus | 25923 | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. Lovett et al (1987) J. Food Protection 50: 188.
2. Fraser and Sperber. 1988. J. Food Prot. 51:762.
3. Refer to the MFDS.
Cat. No : MB-F1166 Fraser Listeria Broth | 500 G |
( 출처 : 기산바이오 )