*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the cultivation and enumeration of a wide variety of microorganisms.
*Equally use with MFDS (MB-T1053K), KP (MB-T1053P), USP, EP and KFCC (MB-T1053C).
CONTENTS (Liter) | Tryptone | 17.0 g |
Soytone | 3.0 g | |
Glucose | 2.5 g | |
Sodium Chloride | 5.0 g | |
Dipotassium Phosphate | 2.5 g | |
Final pH = 7.3 ± 0.2 at 25°C |
Suspend 30.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes.
TSB (Tryptic Soy Broth) is a medium used for the cultivation and enumeration of a wide variety of microorganisms. Tryptone, soytone and dextrose provide nitrogen, carbon, vitamins and minerals. Sodium chloride maintains the osmotic balance. Dipotassium phosphate is the buffering agent.
Inoculate the specimen using a sterile needle to the medium. Incubate at 30 - 35°C for 18 - 24 hours up to 3 days. *Incubate at 20 - 25°C or 30 - 35°C for 18 - 24 hours up to 3 days (**up to 5 days). Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: slight precipitate
Color: light amber
Incubation conditions: 30 - 35°C / 18 - 24 hours up to 3 days
*20 - 25°C or 30 - 35°C / 18 - 24 hours up to 3 days (**up to 5 days)
Microorganism | ATCC | Inoculum CFU | Growth |
Staphylococcus aureus | 6538 | 50-100 | good |
Pseudomonas aeruginosa | 9027 | 50-100 | good |
Salmonella typhimurium | 14028 | 50-100 | good |
Escherichia coli | 8739 | 50-100 | good |
*Bacillus subtilis | 6633 | 50-100 | good |
**Candida albicans | 10231 | 50-100 | good |
**Aspergillus brasiliensis | 16404 | heavy | good |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. Association of Official Analytical Chemists. (1995). Bacteriological analytical manual 8th ed. AOAC International, Gaithersburg, MD.
2. MacFaddin, J.D. (1985). Media for isolation-cultivation-identification maintenance of medical abcteria, p. 797, vol. 1. Williams & Wilkins, Baltimore, MD.
3. ISO 10560 : 1993.
4. Refer to the MFDS, KP, USP, EP and KFCC.
Cat. No : MB-T1053 TSB (Tryptic Soy Broth) | 500 G |
( 출처 : 기산바이오 )