*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Tetrathionate Broth Base
Specifications |
|||||
---|---|---|---|---|---|
Analyte |
Salmonella spp | ||||
Brand |
NEOGEN Culture Media | ||||
Formula |
Enzymatic Digest of Casein |
2.5 g/L | |||
Enzymatic Digest of Animal Tissue |
2.5 g/L | ||||
Bile Salts |
1.0 g/L | ||||
Calcium Carbonate |
10.0 g/L | ||||
Sodium Thiosulfate |
30.0 g/L | ||||
Grams per Liter |
46.00 | ||||
Liters per 500g |
11.00 | ||||
Mass |
500 g | ||||
Package Dimensions |
4.60 IN x 2.90 IN x 8.60 IN | ||||
Package Weight |
1.35 LB | ||||
Preparation |
1. Dissolve 46 g of the medium in one liter of purified water.
2. Heat with frequent agitation and boil for one minutes to completely dissolve the medium.
3. Cool to 45-50°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base. If preparing solution, add 6 grams Iodine + 5 grams Potassium Iodide in 20 mL of purified water.
4. DO NOT REHEAT AFTER ADDING IODINE SOLUTION. Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use. | ||||
Previous Item Number |
Product was previously known as Acumedia Product: 7241 Tetrathionate Broth Base; and LabM Product: LAB097 Tetrathionate Broth Base APHA |
Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans. Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller’s broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food. Tetrathionate Broth Base, abbreviated as TT Broth Base, is specified in standard methods for Salmonella testing. The FDA, Bacteriological Analytical Manual incorporate Tetrathionate Broth Base as a pre-enrichment medium for detecting Salmonella in food mate