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Modified Oxford Antimicrobic Supplement
Code: 211763
6x10ml(vials)
Formula Per 10 mL Vial |
Colistin Sulfate 10.0 mg Moxalactam 20.0 mg |
Directions
1. Suspend 57.5 g of the powder in 1 L of purified water. Mix thoroughly.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder.
3. Autoclave at 121°C for 10 minutes. Cool to 45-50°C.
4. To prepare Oxford Medium:
Dissolve acriflavine (5.0 mg), cefotetan (2.0 mg), colistin sulfate (20.0 mg), cycloheximide(400.0 mg)
and fosfomycin (10.0 mg) in 5 mL reagant grade ethanol and 5 mL purified water.
Filter sterilize supplement before use. Add 10 mL of antimicrobic supplement to 1 L of
molten Oxford Medium Base (45-50°C). Mix thoroughly.
To prepare Modified Oxford Medium:
Aseptically rehydrate one vial of Modified Oxford Antimicrobic Supplement with 10 mL of
sterile purified water. Rotate in an end-over-end motion to dissolve the contents completely.
Add 10 mL of rehydrated Modified Oxford Antimicrobic Supplement to 1 L of Oxford Medium Base
(45-50°C). Mix thoroughly.
5. Test samples of the finished product for performance using stable, typical control cultures.