*** 네이버페이 구매 시 제주도 지역 추가 배송비 안내 ***
네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of coliform microorganisms in food, water and dairy products. *Equally use with MFDS (MB-B1030K), QIA (MB-B1030Q), NIER (MB-B1030N) and Fluoro BGLB (BGLB-MUG) (MB-F1030).
CONTENTS (Liter) | Peptone | 10.0 g |
Lactose | 10.0 g | |
Oxgal | 20.0 g | |
Brilliant Green | 0.0133 g | |
Final pH = 7.2 ± 0.2 at 25°C |
Suspend 40.01 G of powder in 1 L of distilled or deionized water. If necessary, add 1 vial of MUG supplement (MB-M1820) (BGLB-MUG). Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes with durham tubes
1vial contents (each vial is sufficient for 1 L of medium) | |
MUG | 0.05 g |
Rabbit Plasma | 2.5g |
(4-Methylumbelliferyl-β-D-glucuronide) |
BGLB 2% (Brilliant Green Lactose Bile) Broth is a medium used for the the isolation and cultivation of coliform microorganisms in food, water and dairy products. Peptone provides the essential nutrients for growth of organisms. Lactose is the fermentable carbohydrate and lactose fermenting organisms produce gas. Oxgall and brilliant green inhibit Gram-positive bacteria. MUG (4-Methylumbelliferyl-β-D-glucuronide) is a fluorescent substrate for β-D-glucuronidase encoded by Escherichia coli. The enzyme releases a highly fluorescent compound called 4-methylumbelliferone which fluoresces blue when the growths are observed under a UV
Inoculate the specimen with stab using a sterile needle to the medium. Incubate at 35 ± 2°C for 18 - 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light green
Prepared medium
Appearance: clear
Color: green
Incubation conditions: 35 ± 2°C / 18 - 48 hours
Microorganism | ATCC | Inoculum CFU | Growth | Gas |
Escherichia coi | 25922 | 50-100 | good | + |
Enterobacter aerogenes | 13048 | 50-100 | good | + |
Staphylococcus aureus | 25923 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.
1. APHA 1985 “Standard methods for the examination of water and washewater”, 16th edition.
2. ISO 4831 “Microbiology: general giudance for the enumeration of coliforms. Most Probable Number technique” . 1991-03-01
3. FDA (1995) Bacteriological analytical manual, 8th ed.1998. Published by AOAC International.
4. Refer to the MFDS, QIA and NIER.
Cat. No : MB-B1030 BGLB 2% (Brilliant Green Lactose Bile) Broth | 500 G |
( 출처 : 기산바이오 )