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Fraser Listeria Broth [ MB-F1166 ]

리스테리아 2차 증균배지

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Medium used for the isolation and identification of Listeria spp. *Equally use with MFDS (MB-L1166K).

• PROCEDURE

Suspend 54.95 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Aseptically add 2 vials of Fraser Listeria Broth supplement A (MB-F1828) and 2 vials of Fraser Listeria Broth supplement B (MB-F0803). Mix well. Pour into tubes

Fraser Listeria Broth supplement A

Fraser Listeria Broth supplement B

• INTERPRETATION

Fraser Listeria Broth is a medium used for the isolation and identification of Listeria spp. Tryptose, beef extract and yeast extract provide carbon and nitrogen sources and the cofactors required for good growth of Listeria spp. The high concentration of sodium chloride in the medium inhibits the growth of enterococci. Disodium hydrogen phosphate and monopotassium phosphate are the buffering agents. Listeria species hydrolyze esculin. Selectivity is provided by lithium chloride, nalidixic acid and acriflavine HCl. Ferric ammonium citrate is used to detect sulfite reduction. Fraser Listeria Broth except ferric ammonium citrate can be used for the cultivation of Listeria spp.

• TECHNIC

Inoculate the specimen using a sterile needle to the medium. Incubate at 36 ± 1°C for 24 - 48 hours. Refer appropriate references for recommended test procedure.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: beige
Prepared medium
Appearance: slightly opalescent
Color: light amber
Incubation conditions: 36 ± 1°C / 24 - 48 hours

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.

• REFERENCES

1. Lovett et al (1987) J. Food Protection 50: 188.

2. Fraser and Sperber. 1988. J. Food Prot. 51:762.

3. Refer to the MFDS.

• PACKAGE

( 출처 : 기산바이오 )