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네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the isolation and cultivation of Listeria monocytogenes.
*Equally use with MFDS (MB-2192K) and QIA (MB-2192Q)
CONTENTS (Liter) | Pancreatic Digest of Casein | 17.0 g |
Enzymatic Digest of Soybean Meal | 3.0 g | |
Dextrose | 2.5 g | |
Sodium Chloride | 5.0 g | |
Dipotassium phosphate | 2.5 g | |
Yeast Extract | 6.0 g | |
Cycloheximide | 0.05 g | |
Acriflavine HCl | 0.015 g | |
Nalidixic Acid | 0.04 g | |
Final pH = 7.3 ± 0.2 at 25°C |
Suspend 36.1 G of powder in 1L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes.
Listeria Enrichment Broth is a medium used for the isolation and cutivation of Listeria monocytogenes. Pancreatic digest of casein, enzymatic digest of soybean meal and yeast extract provide nitrogen, vitamins and minerals. Dextrose is a carbohydrate source. Sodium chloride maintains the osmotic balance. Dipotassium phosphate is a buffering agent. Cycloheximide is used to inhibit growth of saprophytic fungi. Acriflavine HCl suppresses growth of gram-positive bacteria. Nalidixic acid inhibits growth of gram-negative organisms.
Inoculate the specimen with stab using a sterile needle to the medium. Shake gently for spreading microorganism. Incubate at 30 ± 2°C for 18 - 48 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: clear to slightly opalescent
Color: light yellow
Incubation conditions: 30 ± 2°C / 18 - 48 hours
Microorganism | ATCC | Inoculum CFU | Growth |
Listeria monocytogenes | 15313 | 50-100 | good |
Staphylococcus aureus | 25923 | ≥10³ | inhibited |
Saccharomyces cerevisiae | 76625 | ≥10³ | inhibited |
Escherichia coli | 25922 | ≥10³ | inhibited |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
1. Lovett et al (1987) j. food Protection 50: 188.
2. Refer to the MFDS and QIA.
Cat. No : MB-L2192 Listeria Enrichment Broth | 500 G |
( 출처 : 기산바이오 )