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※ 반품 및 교환 불가 ※

Listeria Enrichment Broth Base (Lovett) [ MB-L1326 ]

리스테리아 증균배지 서플 미첨가

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Broth for enrichment of Listeria spp. according to Lovett's formulation

• DIRECTIONS

Suspend 36.0 G of powder in 1 L of distilled or deionized water. Heat until completely dissolved. Sterilize in autoclave at 121 °C for 15 minutes. Cool to 45-50°C. Aseptically add 2 vials of Listeria Enrichment supplement (MB-L0704). Dispense into final containers.

Listeria Enrichment supplement

• DESCRIPTION

Listeria Enrichment Broth Base (Lovett) is a broth for enrichment of Listeria spp. according to Lovett's formulation.

• TECHNIC

- Add 25 mL of liquid or 25 G of solid test material to 225 mL of Listeria Enrichment Broth Base (Lovett) and mix or blend thoroughly.

- Incubate at 30 ± 2°C for 48 hours.

- Using a sterile loop spread the incubated broth sample onto the entire surface of a selective agar plate for Listeria spp.

- Incubate at 36 ± 1 °C for 24-48 hours.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: clear to slightly opalescent
Color: light yellowish-amber
Incubation conditions: 30 ± 2°C / 18-48 hours

• PRECAUTIONS

The product is not classified as hazardous by current legislation and does not contain harmful substances in concentrations of ≥1%. The product must be used only by properly trained operators.

• STORE

The powder is very hygroscopic: store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared media at 2-6°C.

• REFERENCES

1. International Dairy Federation. 1995. Milk and Milk Products – Detection of Listeria monocytogenes. No 143A. Brussels, Belgium.

2. Hitchens, 1995. In FDA bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.

• PACKAGE

( 출처 : 기산바이오 )