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RVS (Rappaport-Vassiliadis Soya Peptone) Broth [ MB-R0914 ]

살모넬라 2차 증균배지

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Medium used for the isolation and cultivation of Salmonella spp. from meat, dairy products, feces and sewage polluted water.
*Equally use with MFDS (MB-R0914K).

• PROCEDURE

Suspend 26.76 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 115°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes.

• INTERPRETATION

RVS (Rappaport-Vassiliadis Soya Peptone) Broth is a medium used for the isolation and cultivation of Salmonella spp. from meat, dairy products, feces and sewage polluted water. Soya peptone is the carbon and nitrogen sources. Sodium chloride maintains the osmotic balance. Potassium dihydrogen phosphate and dipotassium phosphate are the buffering agents. Magnesium chloride anhydrous makes the medium high osmotic pressure. Malachite green oxalate inhibits many Gram-positive bacteria.

• TECHNIC

Inoculate the specimen using a sterile needle to the medium. Incubate at 41.5 ± 1°C for 20 - 24 hours. Refer appropriate references for recommended test procedure.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: greenish light beige
Prepared medium
Appearance: clear
Color: blue
Incubation conditions: 41.5 ± 1°C / 20 - 24 hours

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.

• REFERENCES

1. Van Schothorst M. and Renaud A.M. (1983) J. Appl. Bact. 54. 209-215.

2. Van Schothorst M., Renauld A. and VanBeek C., 1987, Food Microbiol., 4:11.

3. Rappaport F., Konforti N. and Navon B. (1956) J. Clin. Path. 9. 261-266.

4. Vassiliadis P., Pateraki E., Papaiconomou N., Papadakis J.A. and Trichopoulos D. (1976a) Annales de Microbiologie (Institut Pasteur) 127B. 195-200.

5. Vassiliadis P., Trichopoulos D., Kalapothaki V. and Serie C. (1981) J. Hyg. Camb. 87. 35-39.

6. Harvey R.W.S., Price T.H. and Xirouchaki E. (1979) J. Hyg. Camb. 82. 451-460.

7. Vassiliadis P. (1983) J. Appl. Bact. 54. 69-75.

8. Vassiliadis P., Kalapothaki V., Trichopoulos D., Mavromatte C. and Serie C. (1981) Appl. & Environ. Microbiol. 42. 615-618.

9. Vassiliadis P. (1983) J. Appl. Bact. 56. 69-76.

10. Vassiliadis P., Kalapothaki V. and Trichopoulos D. (1991) J. Food Prot. 54. 421-423.

11. British Pharmacopoeia, 2012, The Stationery office British Pharmacopoeia.

12. Refer to the MFDS.

• PACKAGE

( 출처 : 기산바이오 )