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MYP (Mannitol Egg Yolk Polymyxin) Agar [ MB-M1390 ]

바실러스 세레우스 분리배지

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Medium used for the isolation and cultivation of Bacillus cereus from foods.
*Equally use with MFDS (MB-M1390K), QIA (MB-M1390Q) and Bacillus Cereus Agar (Mossel) (MB-B1114)

• PROCEDURE

Suspend 46.03 G of powder in 900 mL of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Aseptically add 50 ml of Egg Yolk Emulsion (MB-E1864) and 2 vials of Polymyxin B supplement (MB-P0816). Mix well. Pour into petri dishes.

Polymyxin B supplement

• INTERPRETATION

MYP (Mannitol Egg Yolk Polymyxin) Agar is a medium used for the isolation and cultivation of Bacillus cereus from foods. Beef extract and peptone provide nitrogen, carbon and vitamin source. Mannitol fermentation can be detected with phenol red. Sodium chloride maintains osmotic balance. Egg yolk emulsion helps in differentiation of lecithinase producing colonies which are surrounded by a zone of white precipitate. Polymyxin B restricts the growth of Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa.

• TECHNIC

Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 30 ± 2°C for 18 - 48 hours. Refer appropriate references for recommended test procedure.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: pink
Prepared medium
Appearance: slightly opalescent
Color: red-orange
Incubation conditions: 30 ± 2°C / 18 - 48 hours

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration are evident. Store prepared medium at 2-8°C

• REFERENCES

1. Mossel, Koopman and Jongerius. 1967. Appl. Microbiol. 15:650.

2. Donovan. 1958. J. Appl. Bacteriol. 21:100.

3. Coliner. 1948. J. Bacteriol. 55:777.

4. Rhodehamel and Harmon. 1995. FDA bacteriological analytical manual, 8th ed. AOAC International, Gaithersburg, Md.

5. Refer to the MFDS and QIA.

• PACKAGE

( 출처 : 기산바이오 )