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네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Medium used for the wide variety of microorganisms cultivation
CONTENTS (Liter) | Tryptose | 17.0 g |
Soytone | 3.0 g | |
Glucose | 2.5 g | |
Sodium Chloride | 5.0 g | |
Dipotassium Phosphate | 2.5 g | |
Agar | 15 g | |
Final pH = 7.3 ± 0.2 at 25°C |
Suspend 45.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45-50°C in water bath. Mix well. Dispense in petri dishes.
TSA (Tryptic Soy Agar) (MFDS) is a medium used for the wide variety of microorganisms cultivation. Tryptose, soytone and glucose provide nitrogen, carbon, vitamins and minerals. Sodium chloride maintains osmotic balance. Dipotassium phosphate is buffering agents. Agar is the solidifying agent.
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 30 - 35°C for 18 - 24 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: clear with no precipitate
Color: amber
Incubation conditions: 30 - 35°C / 18 - 24 hours
Microorganism | ATCC | Inoculum CFU | Growth |
Escherichia coli | 25922 | 50-100 | good |
Staphylococcus aureus | 25923 | 50-100 | good |
Pseudomonas aeruginosa | 27853 | 50-100 | good |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.
1. Swanson, K.J., F.F. Busta, E.H. Peterson, and M.G. Johnson. (1992). Colony Count Methods, p. 75-95.
2. The United States Pharmacopeia . (1995). Microbiological tests, p. 1681- 1686. The United States pharmacopeia, 23rd Ed. United States Pharmacopeial Convention, Rockville, MD.
3. Refer to the MFDS.
Cat. No : MB-T1052K TSA (Tryptic Soy Agar) (MFDS) | 500 G |
( 출처 : 기산바이오 )