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TSB (Tryptic Soy Broth) [ MB-T1053 ]

일반세균 실험배지

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Medium used for the cultivation and enumeration of a wide variety of microorganisms.
*Equally use with MFDS (MB-T1053K), KP (MB-T1053P), USP, EP and KFCC (MB-T1053C).

• PROCEDURE

Suspend 30.0 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. Mix well. Pour into tubes.

• INTERPRETATION

TSB (Tryptic Soy Broth) is a medium used for the cultivation and enumeration of a wide variety of microorganisms. Tryptone, soytone and dextrose provide nitrogen, carbon, vitamins and minerals. Sodium chloride maintains the osmotic balance. Dipotassium phosphate is the buffering agent.

• TECHNIC

Inoculate the specimen using a sterile needle to the medium. Incubate at 30 - 35°C for 18 - 24 hours up to 3 days. *Incubate at 20 - 25°C or 30 - 35°C for 18 - 24 hours up to 3 days (**up to 5 days). Refer appropriate references for recommended test procedure.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light beige
Prepared medium
Appearance: slight precipitate
Color: light amber
Incubation conditions: 30 - 35°C / 18 - 24 hours up to 3 days
*20 - 25°C or 30 - 35°C / 18 - 24 hours up to 3 days (**up to 5 days)

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C.

• REFERENCES

1. Association of Official Analytical Chemists. (1995). Bacteriological analytical manual 8th ed. AOAC International, Gaithersburg, MD.

2. MacFaddin, J.D. (1985). Media for isolation-cultivation-identification maintenance of medical abcteria, p. 797, vol. 1. Williams & Wilkins, Baltimore, MD.

3. ISO 10560 : 1993.

4. Refer to the MFDS, KP, USP, EP and KFCC.

• PACKAGE

( 출처 : 기산바이오 )