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XLD (Xylose Lysine Desoxycholate) Agar [ MB-X1060 ]

살모넬라 선택배지

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Medium used for the isolation of Salmonella spp. from pharmaceutical products.
* Equally use with MFDS (MB-X1060K), QIA (MB-X1060Q) and NIER (MB-X1060N).

• PROCEDURE

Suspend 56.93 G of powder to 1 L of destilled or deionized water. Heat to boiling until completely dissolved. DO NOT AUTOCLAVE. Cool to 45-50°C in water bath. Mix well. Dispense in petri dishes.

• INTERPRETATION

XLD (Xylose Lysine Desoxycholate) Agar is a selective medium used for the isolation of Salmonella spp. from pharmaceutical products. Xylose, lactose and sucrose are sources of fermentable carbohydrates. Sugar fermentation produces acid, phenol red indicator to change its color to yellow. Sodium chloride provides osmotic balance. Yeast extract provides nitrogen and vitamins. Sodium desoxycholate is inhibitory to gram-positive microorganisms. Sodium thiosulfate and ferric ammonium citrate are components of H2S indicator system. It is included for the visualization of hydrogen sulfide produced, resulting in the formation of colonies with black centers.

• TECHNIC

Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 35 – 37 °C for 24 ± 2 hours. Refer appropriate references for recommended test procedure.

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light pink
Prepared medium
Appearance: slightly opalescent
Color: light red
Incubation conditions: 35 - 37 °C / 24 ± 2 hours

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2-8°C.

• REFERENCES

1. . Taylor, W.I. (1965). Am. J. Clin. Pathol. 44(4) : 471-475.

2. Pollock, H.M., and B.J. Dahlgren (1974). Appl. Microbiol. 27(1): 197-201.

3. Refer to the MFDS, the QIA and the NIER.

• PACKAGE

( 출처 : 기산바이오 )