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MacConkey Sorbitol Agar [ MB-M1108 ]

장출혈성대장균 선택배지

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Medium used for the isolation and cultivation of Escherichia coli O157: H7.
*Equally use with MFDS (MB-M1108K), NIER (MB-M1108N) and TC-SMAC (Tellurite Cefixime-Sorbitol MacConkey Agar) (MB-T0883K).

• PROCEDURE

Suspend 50.03 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. Sterilize by autoclave at 121°C for 15 minutes. Cool to 45 - 50°C in water bath. If necessary, aseptically add 2 vials of C.T. (Cefixime Tellurite) supplement (MB-C0760) (TC-SMAC Agar). Mix well. Pour into petri dishes.

C.T. (Cefixime Tellurite) supplement**

• INTERPRETATION

MacConkey Sorbitol Agar is a medium used for the isolation and cultivation of Escherichia coli O157: H7. Peptone and proteose peptone are the nitrogen and vitamin sources. D-Sorbitol is the fermentable carbohydrate. E.coli strains ferment sorbitol and produce red colonies while E.coli O157 doesn’t ferment sorbitol and produces colorless colonies. Bile salts and crystal violet are selective agents that inhibit the growth of Gram-positive organisms. Sodium chloride maintains the osmotic balance. Neutral red acts as a pH indicator. Agar is the solidifying agent. C.T. (Cefixime Tellurite) supplement makes the medium more selective.

• TECHNIC

Inoculate the specimen using a sterile loop to the medium. Incubate at 36 ± 1°C for 18 - 24 hours. Refer appropriate references for recommended test procedure

• QUALITY CONTROL FOR USE

Dehydrated medium
Appearance: free-flowing, homogeneous
Color: pink-beige
Prepared medium
Appearance : slightly opalescent
Color: red-purple
Incubation conditions: 36 ± 1°C / 18 - 24 hours

• STORE

The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label. Store prepared medium at 2 - 8°C

• REFERENCES

1. Adams, S. (1991). Clinical Lab Science 4(1): 19-20.

2. Rappaport, F., and E. Henig. (1952). J. Clin. Pathology.5: 361-362.

3. Refer to the MFDS and the NIER.

• PACKAGE

( 출처 : 기산바이오 )