English![[NEOGEN]Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA) (98566)](http://bnfkorea.net/web/product/big/202202/9b0f191d2542eb1de7db480fdea6e83b.jpg)
[NEOGEN]Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA) (98566)
[해외배송 가능상품]| 스토어 정보 |
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| 상품명 | [NEOGEN]Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA) (98566) |
|---|---|
| 상품요약정보 | NCM1004 |
| 자체상품코드 | B-98566 |
| 판매가 | ₩173,800 (VAT 포함) |
| ★입고예정일 | 주문시 4주 소요예정! |
| 수량 |  
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| 적립금 | 1,730원 |
| 구매방법 | |
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| 상품명 | 상품수 | 가격 |
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| [NEOGEN]Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA) (98566) |
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판매자 정보
Harlequin® Listeria Chromogenic Agar according to Ottaviani & Agosti (ALOA)
| Specifications | |||||
|---|---|---|---|---|---|
| Analyte | Listeria spp | ||||
| Brand | NEOGEN Culture Media | ||||
| Formula | Meat Peptone | 18.0 g/L | |||
| Tryptone | 6.0 g/L | ||||
| Yeast Extract | 10.0 g/L | ||||
| Lithium Chloride | 10.0 g/L | ||||
| Sodium Chloride | 5.0 g/L | ||||
| Disodium Hydrogen Orthophosphate Anhydrous | 2.5 g/L | ||||
| Sodium Pyruvate | 2.0 g/L | ||||
| Glucose | 2.0 g/L | ||||
| Glycerophosphate | 1.0 g/L | ||||
| Magnesium Sulphate | 0.5 g/L | ||||
| 5-Bromo-4-Chloro-3Indolyl-β-d-Glucopyranoside | 0.05 g/L | ||||
| Agar | 13.5 g/L | ||||
| Grams per Liter | 70.50 | ||||
| Litres per 500g | 7.00 | ||||
| Plates per 500g | 390.00 | ||||
| Package Dimensions | 2.76 IN x 2.76 IN x 3.74 IN | ||||
| Package Weight | 0.31 LB | ||||
| Preparation |
1. Suspend 70.5 g of the medium in 950 mL of purified water.
2. Heat with frequent agitation and boil for 1 minute to completely dissolve the medium. 3. Autoclave at 121°C for 15 minutes. 4. Cool to 45–50°C and add 2 vials of reconstituted NCM4002-0.5* supplement, each reconstituted using 5 mL of sterile deionized/RO water. Swirl to mix. 5. Add 2 vials of NCM4001-0.5* supplement (preheated to 48–50°C). Mix well with gentle end-overend mixing. | ||||
| Previous Item Number | Product was previously known as LabM Product: HAL010 Harlequin® Listeria Chromogenic Agar | ||||
Harlequin® Listeria Chromogenic Agar (according to the formulation of Ottaviani and Agosti) is a selective medium for the isolation and presumptive identification of Listeria monocytogenes from foodstuffs and related materials as described in ISO 11290-1:2017. This medium is not intended to diagnose diseases or other conditions in humans.
Lithium chloride in the base medium and supplementary antimicrobial compounds ceftazidime, polymyxin, nalidixic acid, and cycloheximide provide the medium's selectivity. Chromogenic activity is a result of a chromogenic substrate for the detection of the β-glucosidase enzyme, common to all Listeria spp. and a few strains of Enterococci and Bacilli.
The specific differential activity of this agar is obtained with a proprietary lecithin substrate for the detection of the phospholipase enzyme present in the L. monocytogenes colonies growing on this media. This enzyme activity will result in a halo of precipitation surrounding the target colonies.
Combining both the chromogenic and phospholipase enzyme reactions makes it possible to differentiate Listeria monocytogenes (blue colonies surrounded by an opaque halo) from other Listeria spp. (blue colonies without an opaque halo).
SAFETY DATA SHEET (SDS)
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