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네이버페이 구매 시에는 제주도지역 추가 배송비가 자동적용이 안되기 때문에 추가 배송비를 별도로 입금을 해주셔야 합니다. 기업은행 052-072431-01-014 예금주: ㈜비엔에프코리아, 추가배송비 3,000원 입금부탁드리며, 입금확인 후 출고진행되오니 이점 양해부탁드립니다. ★구매자명으로 입금해주세요~Selective medium used for the isolation of Vibrio cholerae and other pathogenic Vibrio spp. *Equally use with MFDS (MB-T1010K) and QIA (MB-T1010Q).
*Equally use with MFDS (MB-R0914K).
CONTENTS (Liter) | Yeast Extract | 5.0 g |
Peptone | 10.0 g | |
Sodium Citrate | 10.0 g | |
Sodium Thiosulfate | 10.0 g | |
Oxgall | 5.0 g | |
Sodium Cholate | 3.0 g | |
Sucrose | 20.0 g | |
Sodium Chloride | 10.0 g | |
Brom Thymol Blue | 0.04 g | |
Thymol Blue | 0.04 g | |
Agar | 15.0 g | |
Final pH = 8.6 ± 0.2 at 25°C |
Suspend 89.08 G of powder in 1 L of distilled or deionized water. Heat to boiling until completely dissolved. DO NOT AUTOCLAVE. Cool to 45 - 50°C in water bath. Mix well. Pour into petri dishes.
TCBS (Thiosulfate Citrate Bile Salt Sucrose) Agar is used for the selective isolation of Vibrio cholerae and other pathogenic Vibrio from a variety of clinical and nonclinical specimens. Yeast extract and peptone provide nitrogen, carbon, amino acids, minerals and vitamins. Sodium citrate, sodium thiosulfate, oxgall and sodium cholate are selective agents to inhibit gram-positive organisms. Sucrose is the fermentable carbohydrate. Sodium chloride maintains osmotic balance. Ferrous citrate detects hydrogen sulfide production. Brom thymol blue and thymol blue are pH indicators. V. cholerae and V. alginolyticus grow forming yellow colonies because of fermentation of sucrose while V. parahaemolyticus does not ferment sucrose and grows forming blue-green colonies. Agar is solidifying agent.
Inoculate the plates with spreading the specimen on surface of the medium using a sterile loop. Incubate at 35 - 37°C for 18 - 24 hours. Refer appropriate references for recommended test procedure.
Dehydrated medium
Appearance: free-flowing, homogeneous
Color: light tan
Prepared medium
Appearance: slightly opalescent
Color: green
Incubation conditions: 35 - 37°C / 18 - 24 hours
Microorganism | ATCC | Inoculum CFU | Growth | Characteristics |
Vibrio parahaemolyticus | 17802 | 50-100 | good | blue-green colonies |
Vibrio alginolyticus | 17749 | 50-100 | good | yellow colonies |
Escherichia coli | 25922 | ≥10³ | inhibited | - |
The powder is very hygroscopic. Store the powder at room temperature, in a dry environment, in its original container tightly closed and use it before the expiry date on the label or until signs of deterioration or contamination are evident. Store prepared medium at 2 - 8°C.
1. AOAC (1995) – Bacteriological Analytical Manual, 8th ed.
2. Nakanishi, Y. (1963) An isolation agar medium for cholerae and enteropathogenic halophilic vibrios. Modern Media 9: 246.
3. Kobayashi, T., S. Enomoto, R. Sakasaki, and S. Kuwahara (1963). Jap. J. Bacteriol. 18: 10-11; 392-397.
4. Refer to the MFDS and QIA.
Cat. No : MB-T1010 TCBS (Thiosulfate Citrate Bile Salt Sucrose) Agar | 500 G |
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